![]() Alpha adrenergic involvement in heart metabolism: effects on adenosine cyclic 3',5'-monophosphate, adenosine cyclic 3',5'-monophosphate-dependent protein kinase, guanosine cyclic 3',5'-monophosphate, and glucose transport.Characterization of cyclic GMP-binding sites of cyclic GMP-dependent protein kinase by rapid filtration assay.Comparison of cyclic nucleotide binding to adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate-dependent protein kinases.UR - '5'_monophosphate_using_a_binding_protein_from_the_pupa_of_the_silkmoth_Bombyx_mori_L_ Changes in the level of cyclic GMP in the frog heart during the cardiac cycle were determined by means of the new assay. As little as 0.1 pmoles of cyclic GMP can be detected by this procedure. A protein kinase modulator protein isolated from the same species increases the binding affinity and capacity of the cyclic GMP binding protein and can be used to advantage in the assay for cyclic GMP. The dissociation constant of the binding protein for cyclic GMP is 4.3 nM. The assay employs a purified binding protein from the fat body of the pupa of the common silkmoth, Bombyx mori. N2 - A modification of the protein binding assay for cyclic guanosine-3',5'-monophosphate (cyclic GMP) is described that is more sensitive and less subject to interference by cyclic AMP than are previously published protein binding methods. T1 - An improved protein binding assay for guanosine-3',5'-monophosphate using a binding protein from the pupa of the silkmoth, Bombyx mori L.
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